Kosakamoto et al. report that the fly response to protein restriction, including changes in gene expression and food intake, results from a decrease in tyrosine. There are different samples with the same value in multiple figures. The corresponding values in the source data are highlighted in yellow.
Below, to quantify food intake, larvae fed colored food are crushed and absorbance is measured.
Below, it is unclear what the vertical axes mean, but changes in food intake and body weight are shown.
In the source data of Fig. 4f, I found multiple samples having the exact same value as well as cases where one sample has a value that is the exact integer multiple of another sample's value.
The Method section of the paper does not explain how these values were calculated. If the ΔΔCt method was used as in other papers by the same authors, it may explain the occasional appearance of 2x, 4x, 8x values. However, it is hard to interpret the 1:3 pair. In addition, the back-calculated ΔΔCt values of this dataset are infinite decimals, whereas the ΔΔCt values in other papers by the same authors are 2- or 3- digit decimals, which reflects the precision limit of the qPCR machine used. Showing the raw data and the method of calculation will clarify this issue.
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