Figure 2A
COX2 band in COLO-357 and HPAC cells, vertical lines and background that does not fit the rest of the blot. EGFR band in COLO-357 shows and halo and does not fit with the rest. 400X
Figure 6A, EMSA assay (magnify and place bands side by side the corresponding lanes referred below)
1. Control (third lane from left) is the same lane in Gem (nine lane from left). Magnify and match the small dots. The intensity of the NFkappaB band between these two lanes appears "different" but the dots match perfectly.
2. B-DIM, lane 4, matches GEM, lane 8. Note that the small dots match perfectly and also the top of the two bands superimpose exactly. But, interestingly, the NFkappaB band is slightly "different", (darker and rectangular) in GEM from that in B-DIM.
Question related to these EMSA lanes: what are the chances that all imperfections (small dots) in the lanes match perfectly and not the NFkappaB band?
Figure 6C. The EGFR and pEGFR bands in the blot have a peculiar rectangular frame, which does not fit the background and the nature of the technique. 400x